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Registros recuperados: 63
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Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater ArchiMer
Siddique, Mohammad A. Momin; Butts, Ian A. E.; Linhart, Otomar; Macias, Adrian D.; Fauvel, Christian.
Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of...
Tipo: Text Palavras-chave: Egg longevity; Overripe eggs; Reproductive ecology; Mediterranean sea bass; Dicentrarchus labrax.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00279/39000/37580.pdf
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First summary of studies carried out on the grey mullet (Mugil cephalus) in the EU project: diversify ArchiMer
Koven, W; Rosenfeld, H; Fauvel, Christian; Nixon, O; Meiri-ashkenazi, I; Bitan, A; Robles, R; Kotzamanis, Y; Shafran, G; Papaioannou, N.
The deliverable (D7.1) of WP7 “Establishment of a Computer Assisted Sperm Analysis (CASA) for the evaluation of grey mullet sperm” has been submitted. Preliminary analyses show that mullet sperm motility pattern is not similar to that of sea bass (Dicentrarchus labrax) or rainbow trout (Oncorhynchus mykiss) in terms of velocity as well as motility duration (ca 1 min; Fig. 1a). Our preliminary results, indicate that the major bio-technical settings, dilution of sperm, quality of activation and mastering of video recordings have been determined and can now be applied to experimental protocols. Since fish sperm quality is usually assessed through subjective observation, in terms of motility classes and duration, statistical analysis becomes less precise. The...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00307/41776/40991.pdf
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Fitness of early life stages in F1 interspecific hybrids between Dicentrarchus labrax and D. punctatus ArchiMer
Ky, Chin-long; Vergnet, Alain; Molinari, Nicolas; Fauvel, Christian; Bonhomme, Francois.
Inter- and intraspecific crossbreeding experiments were conducted to evaluate the aquaculture potential of hybrids in the genus Dicentrarchus, focusing on fertilisation and hatching success. The experimental design consisted of 24 controlled crosses in which individual and pooled fertilisations were made between wild D. labrax individuals (8 dams and 5 sires originating from West Mediterranean and Atlantic populations) and wild D. punctatus (6 sires). Three experiments were successively performed: (1) dams from the Mediterranean population, individually considered to have good egg quality, (2) dams from Mediterranean population, individually considered to have poor egg quality and (3) crossbreeding using pooled eggs from the Atlantic population. In each...
Tipo: Text Palavras-chave: Sea bass; Hybrid progeny; Offspring quality; Fitness; Dicentrarchus labrax; Dicentrarchus punctatus.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18881/16451.pdf
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Gamete quality and management for in vitro fertilisation in meagre (Argyrosomus regius) ArchiMer
Ramos-júdez, Sandra; González, Wendy; Dutto, Gilbert; Mylonas, Constantinos C.; Fauvel, Christian; Duncan, Neil.
The aquaculture of meagre (Argyrosomus regius) requires methods for the control of reproduction that enable the production of families from specific individuals for selective breeding programs. We experimentally determined the parameters required for an in vitro fertilisation protocol. A total of 14 females and 5 males (mean ± S.D. weights of 20.45 ± 6.22 and 15.94 ± 2.75 kg, respectively) were used. Selected females had vitellogenic oocytes >550 μm in diameter and males had fluid sperm upon application of abdominal pressure. Both sexes were treated with an injection of 15 μg kg−1 of gonadotropin-releasing hormone agonist (GnRHa) to induce oocyte maturation/ovulation and enhance sperm production. To determine the timing of ovulation and window of high...
Tipo: Text Palavras-chave: Argyrosomus regius; Meagre; Reproduction; Artificial fertilisation; Gamete management; GnRHa.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00495/60682/64178.pdf
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Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives ArchiMer
Colleter, Julie; Penman, David J.; Lallement, Stephane; Fauvel, Christian; Hanebrekke, Tanja; Osvik, Renate D.; Eilertsen, Hans C.; D'Cotta, Helena; Chatain, Beatrice; Peruzzi, Stefano.
Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (gamma), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities....
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00239/34996/33531.pdf
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Growth and reproductive performances of juveniles and sexually mature pollack Pollachius pollachius in different conditions ArchiMer
Omnes, Marie-helene; Severe, Armelle; Barone, Herve; Suquet, Marc; Buchet, Vincent; Le Roux, Annick; Gaignon, Jean-louis; Fostier, Alexis; Fauvel, Christian.
The pollack Pollachius pollachius is a commercially important fish, considered as a candidate for aquaculture in France. Rearing attempts are promising (Suquet et al., 1996), and more information is required about its reproduction, to contribute to a better management of breeders for this species. This study was designed to examine the first maturity in pollack and the effects of conditions such as origin and delayed reproduction season, on gonad maturation, reproductive and growth performances of adults.
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Ano: 2002 URL: http://archimer.ifremer.fr/doc/00002/11324/7861.pdf
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Haematological and physiological characteristics of diploid and triploid sea bass, Dicentrarchus labrax L. ArchiMer
Peruzzi, Stefano; Varsamos, Stamatis; Chatain, Beatrice; Fauvel, Christian; Menu, Bruno; Falguiere, Jean-claude; Severe, Armelle; Flik, Gert.
The purpose of this study was to determine whether diploid and triploid sea bass differed in terms of main haematological and physiological characteristics. Diploid and triploid fish were produced by sub-optimal pressure treatments and held in communal environments under standard rearing conditions. Total red blood cell count (RBCC), haemoglobin concentration (Hb), hematocrit (Hct), mean cell volume (MCV), mean cellular haemoglobin content (MCH), mean cell haemoglobin concentration (MCHC), plasma metabolites, osmotic pressure, gill Na+/K+-ATPase activity, electrolytes, cortisol, and 3,5,3'-triiodo-L-thyronine (T-3) were measured and compared. Triploidisation in sea bass led to an increase in erythrocyte size (32% in cytoplasm surface area, and 50% in...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Triploid; Diploid; Physiology; Haematology.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/2005/publication-508.pdf
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Homologous ELISA procedure for the determination of penaeid shrimp vitellogenin ArchiMer
Mendoza, Roberto; Guillaume, Jean-charles; Fauvel, Christian.
Vitellogenin (VTG) was isolated from the haemolymph of Penaeus vannamei by a three step procedure including ultracentrifugation, gel filtration and ion exchange chromatography. VTG was used to raise polyclonal antibodies that were purified by ion exchange chromatography. A two step competitive assay was developed in which VTG could be quantitated by its capacity to inhibit the binding of antibody to the VTG previously adsorbed onto a solid phase. Sensitivity from equilibrium and from non-equilibrium assays was 41 and 2.3 ng/ml respectively. Estimates of within-assay and between-assay variabilities of standard curves were 4.2 and 9.6% respectively. The inhibition curves for dilutions of haemolymph from vitellogenic females, egg yolk extracts and purified...
Tipo: Text Palavras-chave: Pénéidé; Vitellogénine; Vitelline; Immuno-essai (ELISA); Anticorps; Penaeid; Vitellogenin; Vitellin; Immunoassay (ELISA); Antibody.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00188/29894/28333.pdf
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Human chorionic gonadotropin induces spermatogenesis and spermiation in 1-year-old European sea bass (Dicentrarchus labrax): Assessment of sperm quality ArchiMer
Schiavone, R; Zilli, L; Vilella, S; Fauvel, Christian.
The aims of the present study were (a) to compare sperm quality (percentage of motile spermatozoa, motility duration, density and fertility after cryopreservation) between precocious and normally maturing male European sea bass Dicentrarchus labrax, (b) to examine the potential of human chorionic gonadotropin (hCG) to increase spermiation in precocious males and (c) to examine the potential of hCG to induce spermatogenesis and spermiation in non-precocious 1-year-old males. One hundred precocious and 100 non-precocious fish were each randomly divided in two groups each: control (precocious saline-treated and non precocious saline-treated) and treated (precocious hCG-treated and non precocious hCG-treated). Treated groups were administered weekly with 1000...
Tipo: Text Palavras-chave: Dicentrarchus labrax; HCG; European sea bass; Spermiation; Spermatogenesis.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1815.pdf
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Improvement of chilled seabass sperm conservation using a cell culture medium ArchiMer
Fauvel, Christian; Boryshpolets, Sergey; Cosson, J.; Leedy, J. G. Wilson; Labbe, C.; Haffray, Pierrick; Suquet, Marc.
The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00111/22221/20066.pdf
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Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality ArchiMer
Gardes, Lionel; Villanove, Philippe; Buchet, Vincent; Fauvel, Christian.
The present work examines the usefulness of the combination of multivariate analyses and further one-way analysis of variance in the search for links between causes and effects in heterologous stimulation of spawning in fish. Stimulations with both 20 and 50 mu g . kg(-1) doses of [D Trp(6)] luteinizing hormone-releasing hormone analogue (LH-RHa) were effective in inducing and synchronizing spawning of red drum, Sciaenops ocellatus. Oocyte development monitoring using ovarian biopsy allowed treatment injections at different stages of maturation. The influence at stimulation time of female oocyte development state upon further development was shown. An interesting potential of second stimulation, leading to high quality spawns, was established and factors...
Tipo: Text Palavras-chave: Pisciculture; Induction de la ponte; LH-RH analogues; Analyse multivariée; Qualité des oeufs; Développement ovocytaire; Ombrine; Sciaenops ocellatus; Fish culture; Induced breeding; LH-RH analogues; Multivariate analysis; Egg quality; Oocyte development; Red drum; Sciaenops ocellatus.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00187/29875/28361.pdf
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La domestication des Gadidés : le cas de la morue et du lieu jaune ArchiMer
Suquet, Marc; Fauvel, Christian; Gaignon, Jean-louis.
The term "domestication" has a legislative meaning when it applies to species submitted to selection pressures, but also a literary one, according to the time since which the species live closed to men and the possibility of controlling their reproduction. In terms of legislation, none of both species of gadids object of this article, cod and pollack, can be considered as domestic. From a literary point of view, 140 years of research and the control of the reproduction, give to the cod a domestic status. It is not the case with pollack, only studied since ten years and the reproduction of which is still badly controlled. The weak differences observed between the performances of breeding and wild cods focuse the still limited impact of the domestication on...
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Ano: 2004 URL: http://archimer.ifremer.fr/doc/00272/38282/36471.pdf
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La domestication des poissons : le cas du thon rouge ArchiMer
Fauvel, Christian; Suquet, Marc.
Bluefin tuna shows adaptative physiology features allowing life in a wide range of physical and climatic parameters like temperature, salinity and hydrostatic pressure, hence the species fulfil the main basic requirements for progressive domestication and rearing in captivity. The high mortalities recorded after handling and the losses observed after abrupt variations of environment in the cages, emphasised the extreme sensitivity to stress of tuna. As consequences, the progresses about the control of tuna biological cycle are very slow due to limited handling possibilities and tuna aquaculture is restricted to fattening of wild fish. At this time, bluefin tuna production progresses without domestication of the species.
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Ano: 2004 URL: http://archimer.ifremer.fr/doc/00272/38283/36473.pdf
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Marathon vs sprint racers: an adaptation of sperm characteristics to the reproductive strategy of Pacific oyster, turbot and seabass ArchiMer
Suquet, Marc; Cosson, J.; Donval, Anne; Labbe, C.; Boulais, Myrina; Haffray, Pierrick; Bernard, Ismael; Fauvel, Christian.
This work assesses the present knowledge on Pacific oyster sperm biology in comparison to two marine fish species (turbot and seabass) whose sperm characteristics are well described. Sperm morphology mainly differs by the presence of an acrosome in Pacific oyster which is absent in both fish species. In turbot as in Pacific oyster, a sperm maturation process along the genital tract is observed. Sperm motility is triggered by changes in osmolality for seabass and turbot and in pH for Pacific oyster. However, complementary factors are involved to maintain sperm immotile in the genital tract. Sperm movement duration is very long in Pacific oyster (2024 h), compared to turbot (35 min) and seabass (4050 s). A high capacity of ATP regeneration is observed in...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00111/22220/20059.pdf
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Marine fish culture of "new species" farmed in Europe ArchiMer
Suquet, Marc; Divanach, Pascal; Hussenot, Jerome; Coves, Denis; Fauvel, Christian.
In Europe, fish aquaculture in a marine environment developed following two successive waves: Atlantic salmon and then sea-bass and sea-bream. Rearing "new marine fish species" is sustained for many complementary reasons. This paper aims at presenting the aquaculture status of these "new species," research carried out and then problems and perspectives suggested by farming development in these species.
Tipo: Text Palavras-chave: Species; Sea fishes; Fish culture; Europa; Diversification.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6558.pdf
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Marine fish spermatozoa: racing ephemeral swimmers ArchiMer
Cosson, J; Groison, A; Suquet, Marc; Fauvel, Christian; Dreanno, Catherine; Billard, R.
After along period of spermatogenesis (several weeks to months), marine fish spermatozoa are delivered at male spawning in seawater (SW) at the same time as ova. In some fish species, as the ova micropyle closes quickly after release, these minute unicells, the spermatozoa, have to accomplish their task of reaching the micropyle within a very brief period (several seconds to minutes), for delivery of the haploid male genetic information to the ova. To achieve this goal, their high-performance motile equipment, the flagellum, must fully activate immediately on contact with the SW and then propel the sperm cell at an unusually high initial velocity. The cost of such 'hyperactivity' is a very rapid consumption of intracellular ATP that outstrips the supply....
Tipo: Text Palavras-chave: Cell activation; Osmolality; Ions; Cell energetics; Motility; Spermatozoon; Marine fish.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6967.pdf
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Maternally Inherited npm2 mRNA Is Crucial for Egg Developmental Competence in Zebrafish ArchiMer
Bouleau, Aurelien; Desvignes, Thomas; Traverso, Juan Martin; Nguyen, Thaovi; Chesnel, Franck; Fauvel, Christian; Bobe, Julien.
The molecular mechanisms underlying and determining egg developmental competence remain poorly understood in vertebrates. Nucleoplasmin (Npm2) is one of the few known maternal effect genes in mammals, but this maternal effect has never been demonstrated in nonmammalian species. A link between developmental competence and the abundance of npm2 maternal mRNA in the egg was previously established using a teleost fish model for egg quality. The importance of maternal npm2 mRNA for egg developmental competence remains unknown in any vertebrate species. In the present study, we aimed to characterize the contribution of npm2 maternal mRNA to early developmental success in zebrafish using a knockdown strategy. We report here the oocyte- specific expression of npm2...
Tipo: Text Palavras-chave: Egg quality; Fish; Maternal effect gene; Maternal-to-zygotic transition; Nucleoplasmin; Oocyte developmental competence; Teleost; Zygotic genome activation.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00211/32234/30685.pdf
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Motility characteristics of spermatozoa in cod (Gadus morhua) and hake (Merluccius merluccius) ArchiMer
Cosson, Jacky; Groison, Anne-laure; Suquet, Marc; Fauvel, Christian.
As in many marine fish, motility is triggered by osmotic pressure (OP) in cod and hake spermatozoa and motility ranges several minutes with a high velocity (above 50 mu m/s) period restricted to 100 s. A decrease in the flagellar beat frequency (BF) is partly responsible of this briefness. Detailed observations of the flagella during the motility period showed: 1) sea water provokes OP damages (blebs) which impair the correct wave propagation 2) waves become restricted to the proximal flagellum and tip becomes devoid of wave 3) the wave amplitude decreases. The combination of these factors drastically limits to the earliest period of motility the ability for spermatozoa to efficiently progress towards egg for fertilization.
Tipo: Text Palavras-chave: Osmotic pressure; Sperm motility; Spermatozoa; Hake; Cod.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6552.pdf
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NME family and determination of egg quality, new insights from the zebrafish Danio rerio ArchiMer
Desvignes, Thomas; Fauvel, Christian; Fostier, Alexis; Bobe, Julien.
Tipo: Text
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00049/16035/13510.pdf
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Nme protein family evolutionary history, a vertebrate perspective ArchiMer
Desvignes, Thomas; Pontarotti, Pierre; Fauvel, Christian; Bobe, Julien.
Background: The Nme family, previously known as Nm23 or NDPK, is involved in various molecular processes including tumor metastasis and some members of the family, but not all, exhibit a Nucleoside Diphosphate Kinase (NDPK) activity. Ten genes are known in humans, in which some members have been extensively studied. In non-mammalian species, the Nme protein family has received, in contrast, far less attention. The picture of the vertebrate Nme family remains thus incomplete and orthology relationships with mammalian counterparts were only partially characterized. The present study therefore aimed at characterizing the Nme gene repertoire in vertebrates with special interest for teleosts, and providing a comprehensive overview of the Nme gene family...
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6947.pdf
Registros recuperados: 63
Primeira ... 1234 ... Última
 

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